[Cloning and bioinformatics analysis of chorismate mutase gene from Salvia miltiorrhiza].
Identifieur interne : 002419 ( Main/Exploration ); précédent : 002418; suivant : 002420[Cloning and bioinformatics analysis of chorismate mutase gene from Salvia miltiorrhiza].
Auteurs : Ya-Jun Wang [République populaire de Chine] ; Lu-Qi Huang ; Chao Jiang ; Ye ShenSource :
- Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [ 1001-5302 ] ; 2013.
Descripteurs français
- KwdFr :
- Chorismate mutase (composition chimique), Chorismate mutase (génétique), Chorismate mutase (métabolisme), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Phylogenèse (MeSH), Protéines végétales (composition chimique), Protéines végétales (génétique), Protéines végétales (métabolisme), Salvia miltiorrhiza (classification), Salvia miltiorrhiza (composition chimique), Salvia miltiorrhiza (enzymologie), Salvia miltiorrhiza (génétique), Structure tertiaire des protéines (MeSH), Séquence d'acides aminés (MeSH).
- MESH :
- composition chimique : Chorismate mutase, Protéines végétales, Salvia miltiorrhiza.
- enzymologie : Salvia miltiorrhiza.
- génétique : Chorismate mutase, Protéines végétales, Salvia miltiorrhiza.
- métabolisme : Chorismate mutase, Protéines végétales.
- Clonage moléculaire, Données de séquences moléculaires, Phylogenèse, Structure tertiaire des protéines, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Chorismate Mutase (chemistry), Chorismate Mutase (genetics), Chorismate Mutase (metabolism), Cloning, Molecular (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Protein Structure, Tertiary (MeSH), Salvia miltiorrhiza (chemistry), Salvia miltiorrhiza (classification), Salvia miltiorrhiza (enzymology), Salvia miltiorrhiza (genetics).
- MESH :
- chemical , chemistry : Chorismate Mutase, Plant Proteins.
- chemical , genetics : Chorismate Mutase, Plant Proteins.
- chemical , metabolism : Chorismate Mutase, Plant Proteins.
- chemistry : Salvia miltiorrhiza.
- classification : Salvia miltiorrhiza.
- enzymology : Salvia miltiorrhiza.
- genetics : Salvia miltiorrhiza.
- Amino Acid Sequence, Cloning, Molecular, Molecular Sequence Data, Phylogeny, Protein Structure, Tertiary.
Abstract
Chorismate mutase catalyzes the conversion of chorismate to prephenate that is the first committed step in the biosynthesis of the aromatic amino acids phenylalanine and tyrosine. A chorismate mutase gene, designated SmCM1, was isolated from Salvia miltiorrhiza by using RT-PCR. The full length of SmCM1 cDNA consists of 948 nucleotides and has an open reading frame of 765 bp. The deduced amino acid sequence of SmCM1 has 255 amino acid residues which forms a 36.0 kD polypeptide with calculated pI of 6.41 as expected. The putative polypeptide contains a CM_2 super family function domain. Blast W results showed that SmCM1 had 70% of the similarity with Petunia x hybrid CM, 72% of the similarity with Arabidopsis thaliana CM, and 64% of similarity with Populus trichocarpa CM. The transcription level of SmCM1 in root, stem and leaf was analysed by realtime quantitative PCR. The results showed the expression level of the SmCM1 in leaf was highest, and lowest in root. Yeast extract and silver ion joint induction could markedly stimulate the increase of mRNA expression of SmCM1 and its upstream 3-deoxy-7- phosphoheptulonate synthase (DAHPS) and chorismate synthase (CS). It was 7.9, 5.5 and 9.8 times of control on 8 h after induction, respectively.
PubMed: 24010280
Affiliations:
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Jiang</name></author><author><name sortKey="Shen, Ye" sort="Shen, Ye" uniqKey="Shen Y" first="Ye" last="Shen">Ye Shen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:24010280</idno><idno type="pmid">24010280</idno><idno type="wicri:Area/Main/Corpus">002478</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002478</idno><idno type="wicri:Area/Main/Curation">002478</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002478</idno><idno type="wicri:Area/Main/Exploration">002478</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Cloning and bioinformatics analysis of chorismate mutase gene from Salvia miltiorrhiza].</title><author><name sortKey="Wang, Ya Jun" sort="Wang, Ya Jun" uniqKey="Wang Y" first="Ya-Jun" last="Wang">Ya-Jun Wang</name><affiliation wicri:level="1"><nlm:affiliation>National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Huang, Lu Qi" sort="Huang, Lu Qi" uniqKey="Huang L" first="Lu-Qi" last="Huang">Lu-Qi Huang</name></author><author><name sortKey="Jiang, Chao" sort="Jiang, Chao" uniqKey="Jiang C" first="Chao" last="Jiang">Chao Jiang</name></author><author><name sortKey="Shen, Ye" sort="Shen, Ye" uniqKey="Shen Y" first="Ye" last="Shen">Ye Shen</name></author></analytic><series><title level="j">Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica</title><idno type="ISSN">1001-5302</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Chorismate Mutase (chemistry)</term><term>Chorismate Mutase (genetics)</term><term>Chorismate Mutase (metabolism)</term><term>Cloning, Molecular (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Protein Structure, Tertiary (MeSH)</term><term>Salvia miltiorrhiza (chemistry)</term><term>Salvia miltiorrhiza (classification)</term><term>Salvia miltiorrhiza (enzymology)</term><term>Salvia miltiorrhiza (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Chorismate mutase (composition chimique)</term><term>Chorismate mutase (génétique)</term><term>Chorismate mutase (métabolisme)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Salvia miltiorrhiza (classification)</term><term>Salvia miltiorrhiza (composition chimique)</term><term>Salvia miltiorrhiza (enzymologie)</term><term>Salvia miltiorrhiza (génétique)</term><term>Structure tertiaire des protéines (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Chorismate Mutase</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chorismate Mutase</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chorismate Mutase</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Salvia miltiorrhiza</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Salvia miltiorrhiza</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Chorismate mutase</term><term>Protéines végétales</term><term>Salvia miltiorrhiza</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Salvia miltiorrhiza</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Salvia miltiorrhiza</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Salvia miltiorrhiza</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chorismate mutase</term><term>Protéines végétales</term><term>Salvia miltiorrhiza</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Chorismate mutase</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Cloning, Molecular</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Protein Structure, Tertiary</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Phylogenèse</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chorismate mutase catalyzes the conversion of chorismate to prephenate that is the first committed step in the biosynthesis of the aromatic amino acids phenylalanine and tyrosine. A chorismate mutase gene, designated SmCM1, was isolated from Salvia miltiorrhiza by using RT-PCR. The full length of SmCM1 cDNA consists of 948 nucleotides and has an open reading frame of 765 bp. The deduced amino acid sequence of SmCM1 has 255 amino acid residues which forms a 36.0 kD polypeptide with calculated pI of 6.41 as expected. The putative polypeptide contains a CM_2 super family function domain. Blast W results showed that SmCM1 had 70% of the similarity with Petunia x hybrid CM, 72% of the similarity with Arabidopsis thaliana CM, and 64% of similarity with Populus trichocarpa CM. The transcription level of SmCM1 in root, stem and leaf was analysed by realtime quantitative PCR. The results showed the expression level of the SmCM1 in leaf was highest, and lowest in root. Yeast extract and silver ion joint induction could markedly stimulate the increase of mRNA expression of SmCM1 and its upstream 3-deoxy-7- phosphoheptulonate synthase (DAHPS) and chorismate synthase (CS). It was 7.9, 5.5 and 9.8 times of control on 8 h after induction, respectively.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24010280</PMID><DateCompleted><Year>2013</Year><Month>09</Month><Day>24</Day></DateCompleted><DateRevised><Year>2013</Year><Month>09</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1001-5302</ISSN><JournalIssue CitedMedium="Print"><Volume>38</Volume><Issue>11</Issue><PubDate><Year>2013</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica</Title><ISOAbbreviation>Zhongguo Zhong Yao Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Cloning and bioinformatics analysis of chorismate mutase gene from Salvia miltiorrhiza].</ArticleTitle><Pagination><MedlinePgn>1697-702</MedlinePgn></Pagination><Abstract><AbstractText>Chorismate mutase catalyzes the conversion of chorismate to prephenate that is the first committed step in the biosynthesis of the aromatic amino acids phenylalanine and tyrosine. A chorismate mutase gene, designated SmCM1, was isolated from Salvia miltiorrhiza by using RT-PCR. The full length of SmCM1 cDNA consists of 948 nucleotides and has an open reading frame of 765 bp. The deduced amino acid sequence of SmCM1 has 255 amino acid residues which forms a 36.0 kD polypeptide with calculated pI of 6.41 as expected. The putative polypeptide contains a CM_2 super family function domain. Blast W results showed that SmCM1 had 70% of the similarity with Petunia x hybrid CM, 72% of the similarity with Arabidopsis thaliana CM, and 64% of similarity with Populus trichocarpa CM. The transcription level of SmCM1 in root, stem and leaf was analysed by realtime quantitative PCR. The results showed the expression level of the SmCM1 in leaf was highest, and lowest in root. Yeast extract and silver ion joint induction could markedly stimulate the increase of mRNA expression of SmCM1 and its upstream 3-deoxy-7- phosphoheptulonate synthase (DAHPS) and chorismate synthase (CS). It was 7.9, 5.5 and 9.8 times of control on 8 h after induction, respectively.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Ya-Jun</ForeName><Initials>YJ</Initials><AffiliationInfo><Affiliation>National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Lu-Qi</ForeName><Initials>LQ</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Chao</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Ye</ForeName><Initials>Y</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhongguo Zhong Yao Za Zhi</MedlineTA><NlmUniqueID>8913656</NlmUniqueID><ISSNLinking>1001-5302</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 5.4.99.5</RegistryNumber><NameOfSubstance UI="D002826">Chorismate Mutase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002826" MajorTopicYN="N">Chorismate Mutase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="Y">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017434" MajorTopicYN="N">Protein Structure, Tertiary</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D027561" MajorTopicYN="N">Salvia miltiorrhiza</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>9</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>9</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>9</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24010280</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Huang, Lu Qi" sort="Huang, Lu Qi" uniqKey="Huang L" first="Lu-Qi" last="Huang">Lu-Qi Huang</name><name sortKey="Jiang, Chao" sort="Jiang, Chao" uniqKey="Jiang C" first="Chao" last="Jiang">Chao Jiang</name><name sortKey="Shen, Ye" sort="Shen, Ye" uniqKey="Shen Y" first="Ye" last="Shen">Ye Shen</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Ya Jun" sort="Wang, Ya Jun" uniqKey="Wang Y" first="Ya-Jun" last="Wang">Ya-Jun Wang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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